| Encyclopædia Britannica Inc. | book publisher, business | Chicago | 1768 |
| Enstrom Helicopter Corporation | business | Menominee | 1959 |
| Enterprise Products | business, public company | Houston | 1968 |
| Envoy Air | airline | Fort Worth | 1984 |
| Epcot | theme park | — | October 1, 1982 |
| Epicor | business, enterprise | Austin, Irvine | 1972 |
| Epiphone | brand, business | Nashville | 1873 |
| Esalen Institute | retreat center, 501(c)(3) organization, educational institution | Slates Hot Springs | 1962 |
| Esso | brand, gas station chain | Irving | 1911 |
| Estée Lauder Companies | business, enterprise, public company | Neuilly-sur-Seine, New York City | 1946 |
| Eurasia Group | consulting company, business | New York City | 1998 |
| Euronet Worldwide | business, public company | Leawood | 1994 |
| Evergreen State College | liberal arts college, Colleges That Change Lives, public educational institution of the United States | — | 1967 |
| Everts Air | airline | Fairbanks | 1977 |
| Evil Angel | pornographic film studio | Van Nuys | 1989 |
| Evolve | business | Philadelphia | 2009 |
| Exelon | holding company, business, enterprise, public company | Chicago | 2000 |
| Exidy | business | Sunnyvale | 1974 |
| Expedia Group | business, enterprise, public company | Seattle | October 22, 1996 |
| Extreme Championship Wrestling | media franchise, business | Philadelphia | April 25, 1992 |
| Exxon | oil company, business, gas station chain, public company | Las Colinas | January 1, 1973 |
| ExxonMobil | oil company, multinational corporation, business, gas station chain, enterprise, public company | Spring | August 5, 1882 |
| F. W. Woolworth Company | business, public company | New York City | 1878 |
| FactSet | business, public company | Norwalk | 1978 |
| Fairfax County Public Schools | school division of Virginia | — | 1870 |
| Fakku | pornography website, video game publisher, manga publisher | Portland | 2006 |
| Falcon Entertainment | pornographic film distributor, pornography website, pornographic film studio | San Francisco | 1971 |
| Famous Players-Lasky Corporation | film studio | Los Angeles | April 1, 1927 |
| Fandango | online film database, website, business | Los Angeles | 2000 |
| Fantagraphics Books | comics publishing company, book publisher | Seattle | 1976 |
| Faraday Future | automobile manufacturer | Gardena | 2014 |
| Farmers Insurance Group | insurance company, business | Wilshire Boulevard, Los Angeles | 1928 |
| Fatburger | restaurant chain | Santa Monica, Los Angeles | 1952 |
| Faulkner University | church college, private not-for-profit educational institution, university | — | 1942 |
| Fawcett Comics | publishing house | New York City | 1939 |
| Fayetteville State University | historically black college or university, public educational institution of the United States, school, university | Fayetteville | 1867 |
| Feastables | business, company | Chicago | April 2021 |
| Federal Deposit Insurance Corporation | independent agency of the United States government, state-owned enterprise of the United States | Washington, D.C. | June 16, 1933 |
| Federal Law Enforcement Training Center | United States federal agency, police academy, school | Glynco, Georgia | 1970 |
| Federal Reserve Bank of Boston | organization, Federal Reserve Bank | Boston | October 1914 |
| Federal Reserve Bank of Kansas City | organization, Federal Reserve Bank | 1 Memorial Drive, 925 Grand | — |
| Federal Reserve Bank of Philadelphia | organization, Federal Reserve Bank | — | 1914 |
| Feedly | news aggregator | — | 2008 |
| FEI Company | business, enterprise, public company | Hillsboro | 1971 |
| Fender Musical Instruments Corporation | business, musical instrument manufacturing company | Nabari Station | 1946 |
| Fenty Beauty | brand, business | 425 Market Street | September 8, 2018 |
| Fenway Sports Group | business, enterprise | Boston | 2001 |
| Fidelity Investments | business, enterprise | Boston | 1946 |