| National Airlines | airline | Orlando | 1985 |
| National Airlines | airline | Miami-Dade County | 1934 |
| National Catholic Reporter | newspaper, publishing house | Kansas City | 1964 |
| National Center for Biotechnology Information | research institute, data library | Bethesda | November 4, 1988 |
| National Civil Rights Museum | nonprofit organization, history museum, hotel, African-American museum | Memphis | 1968 |
| National Football League | American football league, professional sports league | 345 Park Avenue | 1920 |
| National Gallery of Art | nonprofit organization, publishing house, United States federal agency, public–private partnership, national gallery | Washington, D.C. | March 24, 1937 |
| National Instruments | business, enterprise, public company | Austin | 1976 |
| National Oilwell Varco | business, enterprise, public company, oilfield service company | Houston | 1862 |
| National War College | staff college, military school | Fort Lesley J. McNair | 1946 |
| Naughty America | pornographic film distributor, pornographic film studio | San Diego | 2001 |
| Naval Postgraduate School | naval force university, staff college, public educational institution of the United States, open-access publisher | — | 1909 |
| Naval War College | naval force university, staff college, naval academy | — | 1884 |
| NBC News | production company, online newspaper, television channel, specialty channel | New York City, 30 Rockefeller Plaza | February 21, 1940 |
| Neiman Marcus | business | Dallas | 1907 |
| Neon | film distributor, business | New York | 2017 |
| Nerve Software | video game developer | Richardson | 1999 |
| NetApp | business, enterprise, public company | Sunnyvale | 1992 |
| Netflix, Inc. | television production company, film distributor, business, enterprise, public company | Los Gatos | August 29, 1997 |
| NetherRealm Studios | video game developer | Chicago | 2010 |
| NetJets | airline, business | Columbus | 1964 |
| Neuberger Berman | business, enterprise | New York City | 1939 |
| New Balance | business, subsidiary company | Boston | 1906 |
| New Frontier Hotel and Casino | casino, destroyed building or structure, hotel, music venue | — | 1942 |
| New Holland | business, enterprise | Italy | 1891 |
| New Jersey Transit | transport company | New Jersey, New York City | 1979 |
| New Line Cinema | film production company, film distributor, film studio | Los Angeles | 1967 |
| New World Interactive | video game developer | Denver | 2010 |
| New York Botanical Garden | nonprofit organization, botanical garden, educational institution | The Bronx, New York City | 1891 |
| New York Central Railroad | railway company, business | Helmsley Building | December 23, 1914 |
| New York City Public Schools | municipal authority, school district in the United States, ministry of education | Tweed Courthouse | 2002 |
| New York Film Academy | drama school, film school | New York City | 1992 |
| New York Institute of Technology | private not-for-profit educational institution, university | — | 1955 |
| New York Public Library | nonprofit organization, library network, public library | New York City | May 23, 1895 |
| New York Shipbuilding Corporation | shipbuilding company, business | Camden | 1899 |
| New York University | research university, private not-for-profit educational institution, private university | New York City | 1831 |
| New York University School of Law | law school, faculty, organization | — | June 2, 1835 |
| New York University Tisch School of the Arts | film school, performing arts school | — | 1965 |
| Newegg | website, business | City of Industry | 2001 |
| Newgrounds | dot-com company, user-generated content platform, browser game website, online community, online art gallery | Glenside, Cheltenham Township | July 12, 1995 |
| Nexstar Media Group | media conglomerate, business, public company | Irving | June 17, 1996 |
| NeXT | business, enterprise | Palo Alto, Redwood City | June 20, 1985 |
| Niagara University | private not-for-profit educational institution, university | — | 1856 |
| Nicalis | video game developer | Santa Ana | 2007 |
| Nickel City Opera | opera company | Buffalo | 2004 |
| Nielsen Company | publishing house, business, public company | New York City | 1994 |
| Nightdive Studios | video game publisher, video game developer | Vancouver | 2013 |
| NIQ | business | Chicago | 1923 |