| Darden Restaurants | Orange County | 1968 |
| Databricks | San Francisco | 2013 |
| Datadog | New York City | 2010 |
| Datapoint | — | 1968 |
| DataStax | Santa Clara | April 2010 |
| DC Universe | Burbank | 2018 |
| Dell | Round Rock | September 7, 2016 |
| Dell EMC | Hopkinton | August 1979 |
| Dell Technologies | Round Rock | September 7, 2016 |
| Democratic National Committee | Washington, D.C. | 1848 |
| Detroit Electric Car Company | Detroit | 1907 |
| Devon Energy | Oklahoma City | 1971 |
| Diamond-Star Motors | — | October 1985 |
| Dick's Sporting Goods | Coraopolis | 1948 |
| Dickies | Fort Worth | 1918 |
| DigitalOcean | New York City | August 2011 |
| Diners Club International | Riverwoods | 1950 |
| Discovery, Inc. | New York City | 1982 |
| Disney Cruise Line | Celebration | 1995 |
| Disney Television Animation | Glendale | December 5, 1984 |
| Dolby | San Francisco | 1965 |
| Dollar General | Goodlettsville | 1939 |
| Dominion Energy | Richmond | 1983 |
| DoubleTree by Hilton | McLean | 1969 |
| Dow Jones & Company | New York City | 1882 |
| Dun & Bradstreet | — | 1841 |
| Dunkin' Brands | Canton | 1950 |
| DYMO Corporation | Berkeley | 1958 |
| E-mu Systems | Scotts Valley | 1971 |
| E-ZPass | Wilmington | 1987 |
| E. Remington & Sons | United States | 1816 |
| EA Sports | Burnaby | 1991 |
| EarthLink | Atlanta, Pasadena, Atwater Village | 1994 |
| Eastman Chemical Company | Kingsport | 1920 |
| Eataly | Monticello d'Alba | 2010 |
| Eaton Corporation | Cleveland | 1911 |
| Eclipse Comics | Forestville, Staten Island | 1977 |
| Ecolab | Saint Paul | 1923 |
| Edelman | New York City | 1952 |
| Edmodo | San Mateo | September 1, 2008 |
| Electro-Motive Diesel | La Grange | 1922 |
| Elliott Investment Management | New York City | 1977 |
| Encyclopædia Britannica Inc. | Chicago | 1768 |
| Enstrom Helicopter Corporation | Menominee | 1959 |
| Enterprise Products | Houston | 1968 |
| Epicor | Austin, Irvine | 1972 |
| Epiphone | Nashville | 1873 |
| Estée Lauder Companies | Neuilly-sur-Seine, New York City | 1946 |